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How can i prepare the Nuclease P1 for DNA digestion?
Web1X Nuclease P1 Reaction Buffer 50 mM sodium acetate (pH 5.5 @ 25°C) Storage Buffer. 25 mM Tris-HCl 50 mM NaCl 1 mM ZnCl 2 50% Glycerol pH 7.2 @ 25°C . Heat Inactivation 75°C for 10 minutes Product Notes. Substrate specificity for Nuclease P1 is as follows: 3´ AMP > RNA > ssDNA >> dsDNA. WebJan 1, 2024 · The buffer and RNaseA can also be ordered from Qiagen separately (catalog numbers 19051 and 19101). Buffer P2. 200 mM NaOH. 1% SDS. Buffer P3 (not for spin … fitsnews thomas ravenel
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