WebMar 29, 2024 · 1. Introduction. The yeast transcription factor Gal4 in combination with artificial gene constructs placed under the control of UAS regulatory sequences became a powerful experimental tool when converted into transgenes in other species [1–3].In Drosophila, this binary UAS/Gal4 system has been extensively used for over two … WebJun 17, 2024 · In Drosophila , nutrient status is sensed by the fat body, a functional homolog of mammalian liver and white adipocytes. The fat body conveys nutrient information to insulin-producing cells through humoral factors which regulate Drosophila insulin-like peptide levels and insulin signalling. Insulin signalling has pleiotropic …
The AMPK-PP2A axis in insect fat body is activated by 20 …
WebThe fat body, homologous to human adipocytes and hepatocytes, functions as both an endocrine and energy storage organ; and has been shown to play a critical role in many metabolic processes. WebFat body and the regulation of metabolism. ... Overexpression of AKHR in response to a fat body-specific Gal4 inducer causes dramatic reduction of organismal fat storage. This finding could be recapitulated by fat body-targeted AKHR expression from a cDNA-based upstream activation sequence ... how much to rebuild a diesel engine
Identifying and Characterizing Transgenic Lines That Drive …
WebApr 10, 2024 · To confirm that AMPK indeed inhibits IIS in Drosophila, we genetically manipulated AMPK activity in the fat body of Drosophila larvae using the binary Gal4/UAS system. Driven by a fat body-specific Gal4 … WebAug 24, 2024 · Since the fat body is a multifunctional tissue implicated in energy storage, immune response and a major endocrine organ that secretes proteins governing oocyte maturation, we then tested... WebFeb 7, 2024 · Strikingly, Actβ overexpression in EEs (Pros-Gal4) leads to a dramatic increase in hyperglycemia in a manner dependent on Akh response in the fat body, suggesting inter-organ communication between the midgut and the fat body. Further, using a midgut-specific TKg-Gal4 that targets TK EEs (i.e., about 23% of Actβ-positive EEs), … men\u0027s incline as softshell pants